IVM-M3 (Two-Photon v.3)

The New All-in-One Intravital Imaging Platform

Deep Tissue Imaging, High-resolution, Tunable Laser

IVM-M3 is one of the renowned members of the New All-in-One IVM Series. It has the flexibility feature of the traditional converted microscope and the high-resolution imaging ability of second-harmonic generation microscopy. It is equipped with a fully-automated tunable fs-pulse NIR laser system. IVM-M3 is the optimal system for users who need to conduct deeper-tissue imaging using less-scattering NIR wavelength. Full control functionality of the fs-laser system is integrated with the two-photon imaging software for user convenience with various automation algorithms.

  • • Deep tissue imaging with a tunable long-wavelength NIR fs-laser system
  • • Fully Integrated in vivo Maintenance Unit / Animal Stage (e.g., Monitoring & Homeostatic Regulation of Animal Vitality)
  • • Ultra High-Speed Imaging (max. 100 fps - 512x512 pixels)
  • • 4D Animal Motion Compensation (XYZ & Time)
  • • Label-free, non-linear second and third harmonic generation ability
Liver
Fibrotic collagen
Hepatocyte
Fibrotic collagen
Hepatocyte
Fibrotic collagen
Lipid droplet

Phenotype changes in liver/lung fibrosis model

Specification
IVM-C3 IVM-M3 IVM-CM3 IVM-MS3 IVM-CMS3
Laser
Type Confocal Laser Unit Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Confocal Laser Unit
Tunable Two-Photon Laser Unit

Ti:Sapphire laser
Compact Two-Photon Laser Unit Confocal Laser Unit
Compact Two-Photon Laser Unit
Wavelength 405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
690 – 1,050 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
690-1,050 nm
Fixed 920 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
Fixed 920 nm
Fluorescence
Detector Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Variable Emission Filter
(Optional)
6 or 2 emission filters can be mounted on each of four detectors
ScanHead
Scanner
Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives
Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives
Image
FOV
100 x 100 ㎛ ² – 10 x 10 mm²
Pixel Resolution
Max. 2,048 x 2,048 pixels
Imaging Speed
Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels
In Vivo Animal Stage
3D Stage
Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
4D In Vivo Imaging
Motion Compensation
XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Controllable by IVM Engine software
Additional In Vivo Modules
Live Animal
Maintenance Unit
Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
In Vivo Imaging
Chamber SET
Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Heart Imaging Window SET
Inhalation Anesthesia
System
Rodent Animal Inhalation Anesthesia System
Anesthesia Mask and Connections
Engine & Studio Software
Image Display
Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
In Vivo Imaging Mode
Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)