Repetitive Intravital Imaging
Repetitive Monitoring of Dynamic Phenotype Changes
In Vivo visualization of dynamic molecular & cellular mechanisms
- • Cell differentiation, cell subpopulation
- • Dynamic remodeling of imaging targets
Bone Marrow
Actin
Nuclei
Sinus
30 min
![](/assets/img/applications/a-19.jpg)
84 min
![](/assets/img/applications/a-20.jpg)
Small intestine
Fatty acid
Lacteal
3.5 sec
![](/assets/img/applications/a-22.jpg)
7.3 sec
![](/assets/img/applications/a-23.jpg)
Longitudinal In Vivo Imaging of Cellular-level Dynamic Phenotype Changes Inside the Living Body
- • IVIM Technology’s contract R&D service provides high-quality repetitive observations using imaging chambers.
- • Repetitive intravital imaging of cellular-level dynamics & tissue remodeling inside the living body in vivo will provide you a new insight in the processes of human disease development.
Dynamic Changes in Acute Injury Models
Immune cell dynamics in LPS-induced sepsis
- • Immune cell dynamics in multiple organ dysfunction syndrome
- • Immune cell sequestration in acute respiratory distress syndrome
Lung
0.0 min
Neutrophil
Vessel
![](/assets/img/applications/a-24.jpg)
3.0 min
![](/assets/img/applications/a-25.jpg)
7.5 min
![](/assets/img/applications/a-26.jpg)
![](/assets/img/applications/a-27.jpg)
![](/assets/img/applications/a-28.jpg)
Immune cell behaviors in various ischemia-reperfusion injury models (hepatic / renal / myocardial ischemia-reperfusion, ischemic stroke, hindlimb ischemia)
Liver
Reperfusion 0 h
Neutrophil/Kupffer cell
Hepatocyte
![](/assets/img/applications/a-29.jpg)
Reperfusion 6 h
![](/assets/img/applications/a-30.jpg)
Reperfusion 24 h
![](/assets/img/applications/a-31.jpg)
Immune cell recruitment to irritated spot
- • Cell dynamics in irritated tissue after high-power laser irradiation
- • Wound-healing process at puncture
- • Cell dynamics at toxic chemical-induced injury site
Skin
Nonirradiated
Neutrophil
Vessel
Hair fcllicle
![](/assets/img/applications/a-32.jpg)
Before irradiation
![](/assets/img/applications/a-33.jpg)
6 h after irradiation
![](/assets/img/applications/a-34.jpg)
Dynamic Changes in Chronic Disease Models
Phenotype changes in MCD (methionine and choline deficient)-diet induced NAFLD/NASH (nonalcoholic fatty liver/nonalcoholic steatohepatitis)
- • Hepatic lipid droplet accumulation
- • Immune cell recruitment, hepatocyte necrosis/apoptosis
Liver
Normal diet
Lipid droplet
Vessel
![](/assets/img/applications/a-35.jpg)
MCD Day 2
![](/assets/img/applications/a-36.jpg)
MCD Day 21
![](/assets/img/applications/a-37.jpg)
Phenotype changes in liver/lung fibrosis model
- • Excessive collagen deposition
- • Fibrotic stage (number of septa, etc.)
- • Immune cell recruitment, hepatocyte/alveolar epithelial cell death
Liver
Fibrotic collagen
Hepatocyte
![](/assets/img/applications/a-38.jpg)
Fibrotic collagen
Hepatocyte
![](/assets/img/applications/a-39.jpg)
Fibrotic collagen
Lipid droplet
![](/assets/img/applications/a-40.jpg)
![](/assets/img/applications/a-41.jpg)
Dynamic Changes in Tumor Models
Changes in vascular morphology and vascular permeability
Tumor cell growth, tumor cell death
Tumor cell dissociation
Formation of circulating tumor cells, tumor metastasis
Tumor
Day 7
Vessel
Lung cancer cell
![](/assets/img/applications/a-42.jpg)
Day 10
![](/assets/img/applications/a-43.jpg)
Day 13
![](/assets/img/applications/a-44.jpg)
Vessel morphology
Vessel
Lung cancer cell
![](/assets/img/applications/a-45.jpg)
Vascular permeability
Vessel
Dye
Lung cancer cell
![](/assets/img/applications/a-46.jpg)
Tumor cell dissociation
Breast cancer cell
Stromal cell
![](/assets/img/applications/a-47.jpg)
Dynamic Changes in Brain Disease Models
Changes in BBB penetration
Changes in target accumulation in brain tissue
Changes in cortical capillaries, vascular permeability & vessel diameter
Changes in pericyte coverage
Immune cell recruitment to inflammatory / damaged spot
Tissue cell apoptosis & phagocytosis
Brain
Day 7
Vessel / BBB leakage
![](/assets/img/applications/a-48.jpg)
Day 14
![](/assets/img/applications/a-49.jpg)
Day 21
![](/assets/img/applications/a-50.jpg)
Brain
Sham
Apoptotic body
Vessel
![](/assets/img/applications/a-51.jpg)
Neuroinflammation
Apoptotic body
Vessel
![](/assets/img/applications/a-52.jpg)
![](/assets/img/applications/a-53.jpg)
Brain
Day 4
Neutrophil/Macrophage
Vessel
![](/assets/img/applications/a-54.jpg)
Day 10
![](/assets/img/applications/a-55.jpg)
![](/assets/img/applications/a-56.jpg)